Bowser has another heinous scheme on his claws to defeat Mario and take Princess Peach once and for all, but the mushroom-eating plumber will have some help from six mares accidentally transported to the Mushroom Kingdom by a botched dimension spell.
Twilight learns that the other alicorns have mysteriously disappeared and she is now the last one in the world. Determine to find out what happened to the others, she goes in search of them on an amazing, magical adventure.