Before Twilight will let Apple Bloom try her hoof at another love potion, Apple Bloom has to prove she's ready by mixing other, less potent emotions she and her friends can sample, like fear, rage, embarrassment, stress, and grief.
It's been 25 years since Apple Bloom and her friends found the original Cutie Mark Crusaders, and the C.M.C. is now an Equestrian wide organization, equivalent to the modern day scouting programs.