I'm in a collab with others for Skaltrox Defiance Knight's story of how his character became an anthro version of Princess Twilight sparkle. Her close friends end up as the rest of the mane 6 and other mlp characters.

We need a new rainbow dash, a Fluttershy and Rarity, sweetie belle, apple bloom, babs seed and many more.

anthros of harmony

Taken Characters:
Princess Twilight Sparkle - Skaltrox Defiance Knight
Applejack - kwr2k13
Pinkie Pie - Kikio3000
Rainbow Dash - Chrome Masquerade
Fluttershy - Krushan
Rarity - Calcen
Spike the Dragon - Tempest_Flare
Trixie - The REAL Mister Pkmn
Sunset Shimmer - JimmyHook19
Lyra Heartstrings - Lurkinkon
Octavia Melody - Tide Hunter
Vinyl Scratch/DJ Pon-3 - Tide Hunter
Zecora - Apple Frost
Ember - Dragon Lord - IronHoofes
Shining Armor - Twilight's BBBFF - Fujin777
Starlight Glimmer- Discord the Narrator
Tempest Shadow aka Fizzlepop Berrytwist - shagohad12
Scootaloo - Ephedianroyalty

OCs:
Excalibur Edge - Knightwolf1875
Maestro Spectrum Star -The Real Mister Pkmn
Servo Star
Janice Dazzle Star
Chessmaster Star (Main villain)
Sarah von Solero (Thestral)
Gearhead
Ayumi/Tempest Flare
Alexandra "Alex" Traveler - anthro mouse girl - ScarletRhine

Available:

Tirek (villainous centaur)
Lightning Dust
Reformed Changelings from Thorax's hive
Fleur De Lis
Fancy Pants
Derpy/Ditzy Do
Jet Set
Upper Crust
Berry Punch
Dinky Do
Moondancer
Coloratura/RaRa
Photo Finish
SvenGallop
Gladmane
Iron Will
The Dazzlings (Aria Blaze, Adagio Dazzle, Sonata Dusk)
Sapphire Shores (Singer/Entertainer)
Sassy Saddles (Rarity's business partner)
Spitfire
Celestia and Luna ( the mlp Theory analysts)
Apple Bloom
Sweetie Belle
Babs Seed - cousin of Apple Bloom
Sunflower (Babs Seed's sister)
Gabby the Griffon
Discord (NPC when the plot Is of need of him)
Strawberry Sunrise
Lilly Lace
Starstreak
Inky Rose
Hoity Toity
Soarin
Fleetfoot
Surprise
Wonderbolts (Sky Stinger and Vapor Trail)
Misty Fly
Cranky Doodle Donkey
Matilda
Mr. and Mrs. Cake with their kids, Pound and Pumpkin
Mayor Mare
Filthy Rich
Spoiled Rich
Diamond Tiara
Randolph (The Rich Family Butler)
Photo Finish (Photographer)
Silver Spoon
Twist
Raindrops (Pegasus student)
Cheerilee (a teacher at the school)
Ms. Harshwhinny (another teacher at the school)
Coco Pommel
Fashion Plate
Ms. Peachbottom
Any member of the extended Apple Family
Suri Polomare
Trenderhoof
Quibble Pants
Cheese Sandwich
Bon Bon/Sweetie Drops
The flower trio (florists) Lily, Daisy, and Roseluck
Dr. Whooves
the Buffalo (Little Strongheart and Chief Thunderhooves)
Braeburn
Granny Smith
Apple Rose
Goldie Delicious - Apple Family Historian
Big McIntosh
Former Dragon Lord Torch (Ember's dad)
Principal Abacus Cinch -Crystal Prep administrator
Doughnut Joe
Prince Blueblood
Pina Colada
Sugarcoat
Lemon Zest
Indigo Zap
Sour Sweet
Bulk Biceps
Igneous Rock Pie and Cloudy Quartz
Minuette/Colgate
Twinkle Shine
Lemon Hearts
Spa Ponies (Aloe and Lotus) - good for the spa scene for when Rarity treats her friends for the day spa scenes
Flim and Flam
Princess Cadence - Shining Armor's wife and mother of Flurry Heart
Flurry Heart - foal of Shining Armor and Princess Cadence
Sunburst - Crystal Empire's Crystaler
Crystal Ponies
Silver Shill
Blue Bobbin - Sales Associate of Rarity's
Plaid Stripes
Mr. Stripes - father of Plaid Stripes
Thorax (reformed Changeling)
Pharynx
Reformed Changelings
Queen Chrysalis (Main antagonist) and changeling drones loyal to Chrysalis
Twinkleshine
Lemon Hearts
Cherry Jubilee
Ma Hoofield - Matriarch of the Hoofield Clan
Big Daddy McColt (McColt clan)
Treehugger
Daring Do/A.K. Yearling
Golden Harvest (Carrot farmer)
Gloriosa Daisy(Timber Spruce's sister)
Maud Pie
Juniper Montage
Timber Spruce
Gilda the Griffon
Dr. Callaberon
The Mane-iac
Troubleshoes
Cloudchaser
Flitter
Junebug
Berry Punch
Nurse RedHeart - school nurse
Nurse ponies
Coriander Cumin
Saffron Masala
Zesty Gourmond
Pipsqueak
Zipporwill
Hoofer Step - Dance Instructor
Tender Taps - Student at Ms. Hoofer Step's Dance Studio
Pony Joe - Donut Shop Owner
Fancy Pants
Fleur De Lis
Jet Set
Upper Crust
Clear Skies
Open Skies
Cloudy Skies
Fluffy Clouds
King Sombra - Villain
Sugar Belle
Feather Bangs
Featherweight
Party Favor
Double Diamond
Night Glider
Prince Rutherford - YakYakistan emissary
Yaks - Prince Rutherford's entourage
Any of Photo Finish's Entourage
Snips
Snails
Juniper Montage
Marble Pie
Limestone Pie
Grand Pear (AJ's maternal Grandfather)
Toola Roola
Coconut Crème
Burnt Oak (Firewood Sales Pony)
Davenport - Sofas and Quills Salespony
Cattail
Rumble
Thunderlane
Flitter
Cloudkicker
Sassaflash
Blossomforth
Bow Hot Hoof - Rainbow Dash's dad
Windy Whistles - Rainbow Dash's Mom
Zephyr Breeze - Fluttershy's brother
Mr. and Mrs. Shy - Fluttershy's parents
Mr. Night Light - Twilight and Shining Armor's dad
Mrs. Twilight Velvet - Twilight and Shining Armor's mom
Wallflower Blush
Fuschia Blush
King Sombra (villain)

Pillars of Harmony:

Starswirl the Bearded
Stygian
Somnabula
Mage Meadowbrook
Mistmane
Rockhoof
Flash Magnus

MLP The Movie Characters:

Storm King - villain

Grubber - evil sarcastic sidekick
Capper
Songbird Serenade
Princess Skystar
Queen Novo
Captain Caeleno - Pirate Captain
Captain Caeleno's crew

I believe that's the complete list of characters (including the oc's)