It's winter in Equestria, and Princess Celestia has decided that her best student is in need of a vacation. Twilight Sparkle's five friends all come along as well, as they retire to a tropical seaside paradise, far from Ponyville. Unfortunately, in her exuberance, Pinkie Pie runs off into the dense, uncharted jungle, and the other five quickly go to try and rescue her. But the jungle hold strange, possibly dangerous secrets for them to uncover...

Ambrosia is a young pony who has just gotten her Cutie Mark, a mark that defines her passion in life. The only problem is, no one, not even her, can understand what it is. Ambrosia is dismayed about her strange mark, and doesn't know what to do.