Set a few months after the events of Twilight VS Anime, Twilight is content to relax now that she has seen the appeal in the animation that she thought would once threaten Equestria's way of life and can now focus on her personal life. However, she is called back to duty once more after it has been discovered that anime was suddenly stolen from Equestria's broadcasting stations. Along with the help of her friends, Twilight travels to the birthplace of anime to uncover the identity of the thief and return anime to TV screens across the world.

Once again, Discord could have just snapped his darn fingers and...!