The primary characters of The Glimmerverse are as follows.

Starlight Glimmer: Protege of Princess Celestia

Kind: Unicorn
Element: Magic
Personality: Rational thinking, studious although she does have the occasional OCD moments.
Residence: Canterlot then Golden Oaks Library in Ponyville
Quirks: She is very protective of a lock box that contains the spell of Starswirl's that she altered to create this new universe. She can also be impatient and rather paranoid at times.

Spike: Starlight Glimmer's Assistant

Kind: Dragon
Personality: Generous, knowledgeable and always ready to help.
Residence: Canterlot then Golden Oaks Library in Ponyville

Minuette: Student of Princess Celestia

Kind: Unicorn
Element: Laughter
Personality: Carefree, fun loving and loves her sweets
Residence: Canterlot then Ponyville

Moondancer: Student of Princess Celestia

Kind: Unicorn
Element: Loyalty
Personality: Studious, practical and loyal.
Residence: Canterlot then Ponyville

Lemon Hearts: Student of Princess Celestia

Kind: Unicorn
Element: Honesty
Personality: Practical, honest, hard working
Residence: Canterlot then Sweet Apple Acres
Special Information: In "Closer than She Thinks" in "Starlight Glimmer and Friends" it is discovered that she is Applejack's big sister. She also has earth pony strength and can clear apple trees with one kick.

Lyra Heartstrings: Student of Princess Celestia

Kind: Unicorn
Element: Generosity
Personality: Generous, kind and caring
Residence: Canterlot then Ponyville

Twinkleshine: Student of Princess Celestia

Kind: Unicorn
Element: Kindness
Personality: Kind and caring.
Residence: Canterlot then Ponyville
Special ability: The ability to communicate with and calm down wild animals.

Applejack: Apple farmer and younger sister of Lemon Hearts

Kind: Earth Pony
Personality: Hard working and honest
Residence: Sweet Apple Acres

Princess Celestia: Co-ruler of Equestria

Kind: Alicorn
Personality: Kind, loving, caring and laid back
Residence: Canterlot

Princess Luna: Co-ruler of Equestria

Kind: Alicorn
Personality: Kind, sometimes intense, occasionally emotional.
Residence: Canterlot