These questions are about the protagonists and antagonists of the show. Answers under the spoiler tag.

Who does Spike sleep with a plushie version of?

Rarity

What voice part did Fluttershy initially sing in the Ponytones?

Bass

Which character didn't take a bite of the MMM or investigate?

Applejack

Which character is a year older than Pinkie Pie?

Fluttershy

Which villain almost succeeded in taking over Equestria?

Lord Tirek

Which of the Mane Six was not shown fighting off Ahuizotl's henchponies in Daring Don't?

Rarity

Which two villains share almost identical designs, despite being different species?

Starlight Glimmer and Aria Blaze.

Discord shares his design with which Asian monster?

Chinese dragon.

Which villain was the first to be confirmed as dead in the show?

King Sombra

What type of magic did King Sombra use that was then used in the season four premier?

Alicorn

What was the name of Twilight Sparkle's childhood doll?

Smartypants

Who was the first friend that Twilight Sparkle meets upon her arrival in Ponyville?

Pinkie Pie

How many seconds flat could Rainbow Dash clear the skies in?

Ten seconds

What is the name of Twilight's pet owl?

Owlowiscious

Which of the mane six attended the Grand Galloping Gala in the hopes of getting hitched?

Rarity

Starlight Glimmer uses which of Mage Meadowbrook's magical items?

The Staff of Sameness, but it technically isn't one of them, so...

Which main villain did not get reformed, imprisoned or defeated so far?

Starlight Glimmer