Could Twilight be saved with Human Medical techniques and or tech?
How would the world powers especially the major ones react to the Unicornian Invasion
Would any of them take military action and if so which ones and which ones would lead the coalition against the Unicornian Forces.
Would the countries goes as far as to invade Unicornia after Equestria is liberated if not what what sort of action would be taken against it?
Nukes are a no no

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1. No, not really.

2. Most would not react if they didn't have any ties to Equestria, but if they did, they would condemn the invasion, try to talk them down and if that failed, get ready for any potential invasion. Best reaction would be nations like China, Russia, the UK and the US arming up out of caution.

3. If they weren't attacked, they would stay out of the fight. If Unicornia did attack, they would respond as we humans are very destructive with our toys including our nukes. They would fight initially to see who would lead, but the US would want to be the leader of any coalition.

4. Yes they would. Given our tech, our most destructive action would be to nuke Unicornia's capital to force them into surrender or something like that if it'll have a reduced chance of massive causalities from a normal invasion.

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Not even detox solutions?
Not even if Equestria asked for direct military help?
I wonder what sort of ultimatums the alt real word countries would make
What sort of government does Unicornia use anyways?
Nukes are a no no so other methods must be used.