So guys what is the best way to start a prologue or introduction when starting a RPG/MLP/D&D crossover? Should I start it with Comedy or Random?

Completely depends on the tone you're going with for the rest of the story. The introduction should set the tone for the rest of the story.

6052964
Hehe... Sorry I'm new here, uhh what is this 'Tone' you speak of?

6052991
The 'Tone' of a piece of writing describes what kind of feelings you want to evoke. Is it supposed to be funny? Dark? Scary?

6052997
Funny. But also a little bit of darkness

6053003
Then that is what should be reflected in the introduction. If you have any more questions I might be able to help you.

6053018
Actually I have like a BAZILLION questions, but I guess I can start with three.
1.) Can you maybe.... I don't know, help me with the first story im making?
2.) Proofreading it.. Please?
3.) Maybe help me create some ideas? Or maybe Some inspiration so it can get me to be motivated again. I lost all my motivation and inspiration because my pet dog died four days ago. 🐶😞

I'll help as much as I can, but I am not an author myself. I just read a lot.