Many months had gone by and Twilight worked day and night in the basement, in order to create the most deadly genetically modified virus that she could possibly muster up with her scientific knowledge.
"Is it finished?" the Princess asked.
Twilight Sparkle's horn glowed and she levitated the test tube in the air and showed the Princess. "It's ready," she said.
"How exactly is this going to work?" the Princess asked.
Twilight Sparkle walked over to a giant crate. "Inside this crate are Parasprites. I'm going to inject them with this genetically modified virus and when they bite the ponies, they will receive the disease and die."
"But Parasprites do not bite ponies." The Princess said.
Twilight had a rather sinister grin on her face and said, "But these do! I had your servants travel to the wild tropics and bring me back a special species of Parasprites that do bite ponies, and when they get hungry, they REALLY bite!"
The Princess clapped her front hooves in delight. "Well done Twilight Sparkle, but did you happen to come up with a plan to pretend that we'd be helping ponies?"
Twilight Sparkle nodded. She suddenly took out a giant syringe. Inside the syringe was this green liquid that had a glowing like effect. "I did my Princess," Twilight said. "I have created something that I'd like to call a vaccine. I have filled it with very toxic ingredients that will further damage the health of the ponies. I stole many of Fluttershy's pet animals and did some testing on them. We will begin vaccinating the ponies the second I feel that the virus begins to die off. When ponies stop dying from the outbreak, we can begin the vaccination process and say that our vaccine was the savior and that ponies will flock to us and trust us."
The Princess put her hoof to her chin in thought. "That is such a brilliant idea, Twilight! I knew I could count on you to pull through with this plan."
"So why in Equestria are ya tellin' us that we have to stay indoors fer the next few months? Business has been boomin' on my farm with so many more ponies comin' to Ponyville." Applejack asked.
"I fear that a major catastrophic event will happen soon and I need you girls to stay safe. Science reports suggest that a new virus is going around and it will spread to Ponyville. I need you all to lock yourselves up in your homes and store extra food and water, because I hear it's supposed to be very deadly and many ponies could die."
The rest of Twilight's friends looked at each other with confusion. Each one of them had to make huge sacrifices of their businesses and plans.
"Please take this warning seriously, or the consequences will result in what will most likely be your death. What is about to come to Ponyville, is going to be so horrific and deadly that none of you could possibly imagine the horrors."
Rainbow Dash scratched her head with her hoof. "Then how come you're not going around posting posters and giving speeches about this if it's going to be so deadly?" Rainbow asked.
A tear drop formed in Twilight's eyes and fell to the earth. A tremendous amount of guilt burdened her heart. "I-I-I...." she stuttered. She suddenly turned her back to her friends and said, "I am not allowed to comment on that matter. I have to go now. I will send you all a personal message when I feel it's safe to come back outside again." Twilight Sparkle flared her wings out and took off into the skies.
The rest of her friends sat there on the ground and looked at each other with confusion. They trusted their friend, Twilight and she was a Princess now, so how could she possibly do any harm or evil to others.
"Are you ready to do this?" Twilight asked the Princess.
"Yes, Twilight. I am prepared. Guards! Please come take this crate and unleash the Parasprites outside."
Two guards carried the heavy box over to the door.
Princess Celestia and Twilight Sparkle were upstairs with the guards and Twilight Sparkle used her magic to open the door for the guards. The guards stepped onto the balcony and Twilight closed the door with her magic and locked it shut. Both Princesses stared through the window with curiosity.
The guards balanced the box on the railing and one of them opened up the box. They then shook the box in an attempt to get every last Parasprite out.
Within a matter of seconds, they were bitten by the Parasprites. They took the box off of the railing and placed it aside on the balcony. As they began walking back to enter the room, they suddenly fell to the ground and were shaking with some kind of convulsions. One of the guards began to vomit as he crawled to the door. He put his hoof on the door handle, but he discovered that they locked him out. He banged on it hoping that they would open the door, but the Princesses looked down on the helpless pony and watched him die a painful death, as well as the other stallion.
The first two causalities of this new plague had occurred.
Princess Celestia looked over to Twilight and said, "Twilight, I told you to make it deadly, but I had no idea that it would be THAT deadly."
Twilight shook her head from side to side and said, "Everyponies body is different and may have a different reaction due to their genetic makeup. These guards were some of the strongest and most healthiest ponies we had, but it's possible that the side effects of this virus will not kill off a pony that quickly. I'm rather surprised at the results myself. All we can do now is just sit back and watch what is about to happen to the rest of Ponyville. These Parasites are known to travel quite a distance to feast on flesh."
It didn't take long to hear about the massive casualties from the newly created genetically modified virus.
The media was going nuts and ponies were flocking to the hospitals both young and old. The Doctors had no idea what to do about the case because nopony had ever seen anything like this before. The death rate was climbing so high that the dead had to be carried away in large wagons and they didn't have room or the time to give each pony a personal burial spot. They ended up digging a massive grave and dumping all the bodies down into it.