It's been a hundred years since Discord was resealed in stone by Fausticorn and Twilight and now he's free once again. Alas, he didn't learn his lesson like the two had hoped and now, he's in the real world, behind the My Little Pony writing desk once again creating even more havoc and chaos than before.
Meanwhile in Equestria, Twilight gets word of what Discord has done and transports herself into the human world with her transportation spell once again. This time, though, she transports her friends right along with her. She's sure all six of them will have better luck against Discord than just her Faust last time.
A few moments later, Twilight and her friends land in the human world right outside Hasbro Studios. They then go inside with Twilight and Rarity levitating themselves and Applejack and Pinkie in through a window as Fluttershy and Dash fly up and in the window as well. Once inside, they follow the screaming and soon find Discord torturing the entire show staff. Twilight shoots a beam of magic at him and yells his name to get his attention.
"Discord!" Yells Twilight. "Leave the staff alone! Didn't you learn your lesson from last time?"
"What lesson? Lords of chaos don't do lessons," torts Discord. He then watches a scowl form on Twilight's face as she blasts him with magic once again. "Twilight, do you really think you can ever truly stop me? I'm chaos incarnate!"
Twilight huffs. "If I stopped you last time, we can stop you this time!" She yells. Discord bursts out laughing then out of the corner of his eye, he sees Fluttershy. Twilight backs off and lets her talk to the draconequus instead.
"Why are you doing this? Why are you ruining everything? Why are you risking losing me as a friend? Discord, answer me," she begins to cry, "what fun do you find in making everypony's life a living hell? What fun do you find in making my life a living hell? I thought we were friends. I...I thought we...we were friends." She then bursts into full on tears.
Discord bows his head. He knows Fluttershy is right. What he's doing is messing up her life as much as the lives of those around her yet he still does it anyways. He wants to keep her as a friend, but he can't put his chaotic nature at bay. If he does, he'll disappear forever. Fluttershy knows this fact better than any pony. She does what she has to do. She reluctantly tells Twilight to try to re-encase him in stone even though she hates the very thought of it.